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article 650856  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences article 650856
    Article 650856, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/article+650856/Flow+Cytometry+Perm+Buffer/pm34211460-48-12-20
    Average 97 stars, based on 1 article reviews
    article 650856 - by Bioz Stars, 2026-11
    97/100 stars

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    Staining:

    Article Title: Dietary Supplementation With Eicosapentaenoic Acid Inhibits Plasma Cell Differentiation and Attenuates Lupus Autoimmunity.
    Article Snippet: The following conjugated antibodies were used for the staining: B220 (RA3-6B2), CD23 (B3B4), CD21 (7E9), CD138 (281-2), CD95 (SA367H8), GL7 (GL7), CD3 (145-2C11), CD4 (GK1.5), CD8 (53-6.7), CD11c (N418), MHCII (M5/144.15.2), PDCA (9.27E+02), CD11b (M1/70), Ly6G (1A8), CD44 (IM7), CD62L (MEL-14), PD-1 (29F.1A12), CD25 (3C7) (BioLegend), Peanut Agglutinin (PNA, Vector Laboratories), and DAPI (BioLegend). .. For intracellular staining, cells were incubated June 2021 | Volume 12 | Article 650856 with Transcription Factor Fix/Perm Working Buffer (TONBO Biosciences) at room temperature for 30 min. After washing twice with Flow Cytometry Perm Buffer (TONBO Biosciences), cells were stained with APC-conjugated anti-Blimp1 (5E7, BioLegend) or PE-conjugated anti-Foxp3 (3G3, TONBO Biosciences) at room temperature for 20 min in the dark. .. After washing, cells were analyzed on MACSQuant Analyzer (Miltenyi Biotec) and FlowJo software (BD Bioscience).

    Incubation:

    Article Title: Dietary Supplementation With Eicosapentaenoic Acid Inhibits Plasma Cell Differentiation and Attenuates Lupus Autoimmunity.
    Article Snippet: The following conjugated antibodies were used for the staining: B220 (RA3-6B2), CD23 (B3B4), CD21 (7E9), CD138 (281-2), CD95 (SA367H8), GL7 (GL7), CD3 (145-2C11), CD4 (GK1.5), CD8 (53-6.7), CD11c (N418), MHCII (M5/144.15.2), PDCA (9.27E+02), CD11b (M1/70), Ly6G (1A8), CD44 (IM7), CD62L (MEL-14), PD-1 (29F.1A12), CD25 (3C7) (BioLegend), Peanut Agglutinin (PNA, Vector Laboratories), and DAPI (BioLegend). .. For intracellular staining, cells were incubated June 2021 | Volume 12 | Article 650856 with Transcription Factor Fix/Perm Working Buffer (TONBO Biosciences) at room temperature for 30 min. After washing twice with Flow Cytometry Perm Buffer (TONBO Biosciences), cells were stained with APC-conjugated anti-Blimp1 (5E7, BioLegend) or PE-conjugated anti-Foxp3 (3G3, TONBO Biosciences) at room temperature for 20 min in the dark. .. After washing, cells were analyzed on MACSQuant Analyzer (Miltenyi Biotec) and FlowJo software (BD Bioscience).

    Flow Cytometry:

    Article Title: Dietary Supplementation With Eicosapentaenoic Acid Inhibits Plasma Cell Differentiation and Attenuates Lupus Autoimmunity.
    Article Snippet: The following conjugated antibodies were used for the staining: B220 (RA3-6B2), CD23 (B3B4), CD21 (7E9), CD138 (281-2), CD95 (SA367H8), GL7 (GL7), CD3 (145-2C11), CD4 (GK1.5), CD8 (53-6.7), CD11c (N418), MHCII (M5/144.15.2), PDCA (9.27E+02), CD11b (M1/70), Ly6G (1A8), CD44 (IM7), CD62L (MEL-14), PD-1 (29F.1A12), CD25 (3C7) (BioLegend), Peanut Agglutinin (PNA, Vector Laboratories), and DAPI (BioLegend). .. For intracellular staining, cells were incubated June 2021 | Volume 12 | Article 650856 with Transcription Factor Fix/Perm Working Buffer (TONBO Biosciences) at room temperature for 30 min. After washing twice with Flow Cytometry Perm Buffer (TONBO Biosciences), cells were stained with APC-conjugated anti-Blimp1 (5E7, BioLegend) or PE-conjugated anti-Foxp3 (3G3, TONBO Biosciences) at room temperature for 20 min in the dark. .. After washing, cells were analyzed on MACSQuant Analyzer (Miltenyi Biotec) and FlowJo software (BD Bioscience).

    Cytometry:

    Article Title: Dietary Supplementation With Eicosapentaenoic Acid Inhibits Plasma Cell Differentiation and Attenuates Lupus Autoimmunity.
    Article Snippet: The following conjugated antibodies were used for the staining: B220 (RA3-6B2), CD23 (B3B4), CD21 (7E9), CD138 (281-2), CD95 (SA367H8), GL7 (GL7), CD3 (145-2C11), CD4 (GK1.5), CD8 (53-6.7), CD11c (N418), MHCII (M5/144.15.2), PDCA (9.27E+02), CD11b (M1/70), Ly6G (1A8), CD44 (IM7), CD62L (MEL-14), PD-1 (29F.1A12), CD25 (3C7) (BioLegend), Peanut Agglutinin (PNA, Vector Laboratories), and DAPI (BioLegend). .. For intracellular staining, cells were incubated June 2021 | Volume 12 | Article 650856 with Transcription Factor Fix/Perm Working Buffer (TONBO Biosciences) at room temperature for 30 min. After washing twice with Flow Cytometry Perm Buffer (TONBO Biosciences), cells were stained with APC-conjugated anti-Blimp1 (5E7, BioLegend) or PE-conjugated anti-Foxp3 (3G3, TONBO Biosciences) at room temperature for 20 min in the dark. .. After washing, cells were analyzed on MACSQuant Analyzer (Miltenyi Biotec) and FlowJo software (BD Bioscience).



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    article 650856  (Cytek Biosciences)
    97
    Cytek Biosciences article 650856
    Article 650856, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/article+650856/Flow+Cytometry+Perm+Buffer/pm34211460-48-12-20
    Average 97 stars, based on 1 article reviews
    article 650856 - by Bioz Stars, 2026-11
    97/100 stars
    		  Buy from Supplier

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